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Density gradient centrifugation is known to be one of the most efficient methods for separating suspended particles, and is used both as a separation technique and as a method for measuring the density of particles or molecules in a mixture. [17]
Historically a cesium chloride (CsCl) solution was often used, but more commonly used density gradients are sucrose or Percoll.This application requires a solution with high density and yet relatively low viscosity, and CsCl suits it because of its high solubility in water, high density owing to the large mass of Cs, as well as low viscosity and high stability of CsCl solutions.
The difference between differential and density gradient centrifugation techniques is that the latter method uses solutions of different densities (e.g. sucrose, Ficoll, Percoll) or gels through which the sample passes.
Density gradient is a spatial variation in density over an area. The term is used in the natural sciences to describe varying density of matter , but can apply to any quantity whose density can be measured .
Isopycnic centrifugation refers to a method wherein a density gradient is either pre-formed or forms during high speed centrifugation. After this gradient is formed particles move within the gradient to the position having a density matching their own (this is in fact an incorrect description of the exact physical process but does describe the ...
Concentration of Plasmodium falciparum-infected erythrocytes by discontinuous density gradient centrifugation in Percoll. [1] Percoll is a reagent consisting of colloidal silica particles used in cell biology and other laboratory settings. It was first formulated by Pertoft and colleagues, [2] and commercialized by Pharmacia Fine Chemicals. [3]
Rate-zonal centrifugation is a centrifugation technique employed to effectively separate particles of different sizes. [1] The tube is first filled with different concentrations of sucrose or another solute establishing layers with different densities and viscosities, forming a density gradient, within which the particles to be separated are added.
Isopycnic centrifugation, often used to isolate nucleic acids such as DNA; Sucrose gradient centrifugation, often used to purify enveloped viruses and ribosomes, and also to separate cell organelles from crude cellular extracts; There are different types of laboratory centrifuges: Microcentrifuges