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  2. GUIDE-Seq - Wikipedia

    en.wikipedia.org/wiki/GUIDE-Seq

    GUIDE-Seq (Genome-wide, Unbiased Identification of DSBs Enabled by Sequencing) is a molecular biology technique that allows for the unbiased in vitro detection of off-target genome editing events in DNA caused by CRISPR/Cas9 as well as other RNA-guided nucleases in living cells. [1]

  3. Pacing (activity management) - Wikipedia

    en.wikipedia.org/wiki/Pacing_(activity_management)

    Pacing has been used to help manage a wide variety of different illnesses and disabilities, including neuromuscular diseases like Charcot-Marie-Tooth disease (CMT), [7] rheumatoid or immune-mediated diseases like rheumatoid arthritis, [6] juvenile arthritis and fibromyalgia, ME/CFS, [8] Ehlers–Danlos syndromes (EDS), [3] and Long COVID. [5]

  4. Sequencing - Wikipedia

    en.wikipedia.org/wiki/Sequencing

    DNA sequencing is the process of determining the nucleotide order of a given DNA fragment. So far, most DNA sequencing has been performed using the chain termination method developed by Frederick Sanger. This technique uses sequence-specific termination of a DNA synthesis reaction using modified nucleotide substrates.

  5. Primer walking - Wikipedia

    en.wikipedia.org/wiki/Primer_walking

    Primer walking is an example of directed sequencing because the primer is designed from a known region of DNA to guide the sequencing in a specific direction. In contrast to directed sequencing, shotgun sequencing of DNA is a more rapid sequencing strategy. [6] There is a technique from the "old time" of genome sequencing.

  6. DNA sequencer - Wikipedia

    en.wikipedia.org/wiki/DNA_sequencer

    The first DNA sequencing methods were developed by Gilbert (1973) [8] and Sanger (1975). [9] Gilbert introduced a sequencing method based on chemical modification of DNA followed by cleavage at specific bases whereas Sanger's technique is based on dideoxynucleotide chain termination. The Sanger method became popular due to its increased ...

  7. Pace (unit) - Wikipedia

    en.wikipedia.org/wiki/Pace_(unit)

    A pace is a unit of length consisting either of one normal walking step (approximately 0.75 metres or 30 inches), or of a double step, returning to the same foot (approximately 1.5 metres or 60 inches).

  8. A Guide to the Scientific Knowledge of Things Familiar

    en.wikipedia.org/wiki/A_Guide_to_the_Scientific...

    A Guide to the Scientific Knowledge of Things Familiar, also known as The Guide to Science or Brewer's Guide to Science, is a book by Ebenezer Cobham Brewer presenting explanations for common phenomena. [1] First published in the United Kingdom around 1840, the book is laid out in the style of a catechism and proved very popular.

  9. Rapid amplification of cDNA ends - Wikipedia

    en.wikipedia.org/wiki/Rapid_amplification_of...

    Rapid amplification of cDNA ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell. RACE results in the production of a cDNA copy of the RNA sequence of interest, produced through reverse transcription, followed by PCR amplification of the cDNA copies (see RT-PCR).

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