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  2. Sequencing - Wikipedia

    en.wikipedia.org/wiki/Sequencing

    DNA sequencing is the process of determining the nucleotide order of a given DNA fragment. So far, most DNA sequencing has been performed using the chain termination method developed by Frederick Sanger. This technique uses sequence-specific termination of a DNA synthesis reaction using modified nucleotide substrates.

  3. List of sequenced eukaryotic genomes - Wikipedia

    en.wikipedia.org/wiki/List_of_sequenced...

    Saccharomyces cerevisiae was the first eukaryotic organism to have its complete genome sequence determined.. This list of "sequenced" eukaryotic genomes contains all the eukaryotes known to have publicly available complete nuclear and organelle genome sequences that have been sequenced, assembled, annotated and published; draft genomes are not included, nor are organelle-only sequences.

  4. RNA-Seq - Wikipedia

    en.wikipedia.org/wiki/RNA-Seq

    The mRNA is extracted from the organism, fragmented and copied into stable ds-cDNA (blue). The ds-cDNA is sequenced using high-throughput, short-read sequencing methods. These sequences can then be aligned to a reference genome sequence to reconstruct which genome regions were being transcribed. This data can be used to annotate where expressed ...

  5. Seq2seq - Wikipedia

    en.wikipedia.org/wiki/Seq2seq

    Shannon's diagram of a general communications system, showing the process by which a message sent becomes the message received (possibly corrupted by noise). seq2seq is an approach to machine translation (or more generally, sequence transduction) with roots in information theory, where communication is understood as an encode-transmit-decode process, and machine translation can be studied as a ...

  6. DNA sequencing - Wikipedia

    en.wikipedia.org/wiki/DNA_sequencing

    Sequencing by ligation (SOLiD sequencing) 50+35 or 50+50 bp: 99.9%: 1.2 to 1.4 billion: 1 to 2 weeks: $60–130: Low cost per base. Slower than other methods. Has issues sequencing palindromic sequences. [109] Nanopore Sequencing: Dependent on library preparation, not the device, so user chooses read length (up to 2,272,580 bp reported [110 ...

  7. ABI Solid Sequencing - Wikipedia

    en.wikipedia.org/wiki/ABI_Solid_Sequencing

    It uses 2 base encoding to decode the raw data generated by the sequencing platform into sequence data. This method should not be confused with "sequencing by synthesis," a principle used by Roche-454 pyrosequencing (introduced in 2005, generating millions of 200-400bp reads in 2009), and the Solexa system (now owned by Illumina) (introduced in ...

  8. Sequencing by hybridization - Wikipedia

    en.wikipedia.org/wiki/Sequencing_by_hybridization

    The type of sequencing by hybridization described above has largely been displaced by other methods, including sequencing by synthesis, and sequencing by ligation (as well as pore-based methods). However hybridization of oligonucleotides is still used in some sequencing schemes, including hybridization-assisted pore-based sequencing, and ...

  9. Spectral sequence - Wikipedia

    en.wikipedia.org/wiki/Spectral_sequence

    In homological algebra and algebraic topology, a spectral sequence is a means of computing homology groups by taking successive approximations. Spectral sequences are a generalization of exact sequences, and since their introduction by Jean Leray (1946a, 1946b), they have become important computational tools, particularly in algebraic topology, algebraic geometry and homological algebra.

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