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A UV-Vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet (UV) and visible light that is absorbed by a sample. It is a widely used technique in chemistry, biochemistry, and other fields, to identify and quantify compounds in a variety of samples.
DU Spectrophotometer, National Technical Laboratories, 1947. The DU spectrophotometer or Beckman DU, introduced in 1941, was the first commercially viable scientific instrument for measuring the amount of ultraviolet light absorbed by a substance.
The Cary 14 spectrophotometer was first produced in 1954 by the Applied Physics Corporation, which later was named the Cary Instruments Corporation after co-founder Howard Cary. [1] The instrument was a successor to the Cary 11, which was the first commercially available recording UV/Vis spectrophotometer. [3]
Ultraviolet-visible (UV-vis) spectroscopy involves energy levels that excite electronic transitions. Absorption of UV-vis light excites molecules that are in ground-states to their excited-states. [5] Visible region 400–700 nm spectrophotometry is used extensively in colorimetry science. It is a known fact that it operates best at the range ...
UV–vis spectroscopy sees only chromophores, so other molecules must be prepared for analysis by chemical addition of a chromophore such as anthracene. Two methods are reported: the octant rule and the exciton chirality method. [1] The octant rule was introduced in 1961 by William Moffitt, R. B. Woodward, A. Moscowitz, William Klyne and Carl ...
English: Controls on UV-vis spectrophotometer : 1-Wavelength selection; 2-Printer button; 3-Concentration factor adjustement; 4-UV mode selector (Deuterium lamp);
If the instrument is designed to measure the spectrum on an absolute scale rather than a relative one, then it is typically called a spectrophotometer. The majority of spectrophotometers are used in spectral regions near the visible spectrum. A spectrometer that is calibrated for measurement of the incident optical power is called a ...
It is the link between the electrochemistry and the UV-Vis absorption spectroscopy. [3] Devices to conduct the radiation beam: lenses, mirrors and/or optical fibers. The last ones conduct electromagnetic radiation over great distances with hardly any losses.
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