Search results
Results from the WOW.Com Content Network
Human AP Endonuclease 2 (APE2), like most AP endonucleases, is also of class II. The exonuclease activity of APE2 is strongly dependent upon metal ions. However, APE2 was more than 5-fold more active in the presence of manganese than of magnesium ions. [4] The conserved domains involved in catalytic activity are located at the N-terminal part ...
The AP endonuclease recognizes this sugar and essentially cuts the DNA at this site and then allows for DNA repair to continue. [10] E. coli cells contain two AP endonucleases: endonuclease IV (endoIV) and exonuclease III (exoIII) while in eukaryotes, there is only one AP endonuclease. [11]
Monofunctional glycosylases have only glycosylase activity, whereas bifunctional glycosylases also possess AP lyase activity that permits them to cut the phosphodiester bond of DNA, creating a single-strand break without the need for an AP endonuclease. β-Elimination of an AP site by a glycosylase-lyase yields a 3' α,β-unsaturated aldehyde ...
The mutated base gets removed through the base excision repair pathway, a two step mechanism carried out by a glycosylase and AP endonuclease. DNA deoxyinosine glycosylase initiates the process through hydrolytic cleavage of the hypoxanthine base, releasing a free hypoxanthine and creating an abasic, or AP site. AP endonuclease follows ...
AP endonucleases are divided into two families based on their homology to the ancestral bacterial AP endonucleases endonuclease IV and exonuclease III. [6] Many eukaryotes have members of both families, including the yeast Saccharomyces cerevisiae , in which Apn1 is the EndoIV homolog and Apn2 is related to ExoIII.
The Grand Canyon in Arizona is one of Earth's natural wonders, carved out over millions of years by the gradual erosion power of the Colorado River. Close to the moon's south pole are two canyons ...
Travis Hunter will arrive at the NFL's annual scouting combine listed as a cornerback. Hunter's emergence as potentially the top defensive back and the top receiver in this year's draft class has ...
All cells, from simple prokaryotes to humans, have evolved systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site, thereby initiating a process known as base excision repair (BER). The APEX gene (alternatively named APE1, HAP1, APEN) encodes the major AP endonuclease in human cells.