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Differentiated adipocytes in a 3T3-L1 cell line stained with Oil Red O. 3T3-L1 is a sub clonal cell line derived from the original 3T3 Swiss albino cell line of 1962. The 3T3 original cell line was isolated from a mouse embryo and propagated for this specific line of 3T3 cells is used to study adipose tissue-related diseases and dysfunctions.
3T3 cells are several cell lines of mouse embryonic fibroblasts. The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green .
Reverse transfection is more expensive because a highly accurate and efficient microarray printing system is needed to print the DNA-gelatin solution onto the slides. Applications with different cell lines have (so far) required protocol variations to manufacture siRNA or plasmid arrays, which involve considerable development and testing.
Tissue nanotransfection (TNT) is an electroporation-based technique capable of gene and drug cargo delivery or transfection at the nanoscale. Furthermore, TNT is a scaffold-less tissue engineering (TE) technique that can be considered cell-only or tissue inducing depending on cellular or tissue level applications.
In genetic engineering, a gene gun or biolistic particle delivery system is a device used to deliver exogenous DNA , RNA, or protein to cells. By coating particles of a heavy metal with a gene of interest and firing these micro-projectiles into cells using mechanical force, an integration of desired genetic information can be introduced into ...
Gene delivery is the process of introducing foreign genetic material, such as DNA or RNA, into host cells. [1] Gene delivery must reach the genome of the host cell to induce gene expression. [2]
Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. [1] It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid DNA into in vitro cell cultures by lipofection. [1]
Hydrodynamic Delivery was developed as a way to insert genes without viral infection (transfection). The procedure requires a high-volume DNA solution to be inserted into the veins of the rodent using a high-pressure needle. [2] The volume of the DNA is typically 8-10% equal to 8-10% of the animal's body weight, and is injected within 5-7 seconds.