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The first few steps of COBRA, and the molecular changes caused by each step to methylated and unmethylated CpG sites. Combined Bisulfite Restriction Analysis (or COBRA) is a molecular biology technique that allows for the sensitive quantification of DNA methylation levels at a specific genomic locus on a DNA sequence in a small sample of genomic DNA. [1]
Cell-free fetal DNA (cffDNA) testing – a non-invasive (for the fetus) test. It is performed on a sample of venous blood from the mother, and can provide information about the fetus early in pregnancy. [12] As of 2015 it is the most sensitive and specific screening test for Down syndrome. [13]
To start off processing in the laboratory, the sample has to first go through an extraction protocol, which may vary depending on the sample type or laboratory SOPs (Standard Operating Procedures). Once the DNA has been "extracted" from the cells within the sample and separated away from extraneous cellular materials and any nucleases that ...
Loop-mediated isothermal amplification (LAMP) primers [1] Loop-mediated isothermal amplification (LAMP) product [1]. In LAMP, the target sequence is amplified at a constant temperature of 60–65 °C (140–149 °F) using either two or three sets of primers and a polymerase like Bst Klenow fragment with high strand displacement activity in addition to a replication activity.
C 0 t analysis, a technique based on the principles of DNA reassociation kinetics, is a biochemical technique that measures how much repetitive DNA is in a DNA sample such as a genome. [1] It is used to study genome structure and organization and has also been used to simplify the sequencing of genomes that contain large amounts of repetitive ...
The Dische test, or Dische reaction, is used to distinguish DNA from RNA. It was invented by Zacharias Dische. Method. ... When heated with DNA, it turns blue in the ...
These include genotoxicity testing, human biomonitoring and molecular epidemiology, ecogenotoxicology, as well as fundamental research in DNA damage and repair. [15] For example, Swain and Rao, using the comet assay [16] reported marked increases in several types of DNA damages in rat brain neurons and astrocytes during aging, including single ...
The branched DNA binds to the sample nucleic acid by specific hybridization in areas which are not occupied by capture hybrids. The branching of the DNA allows for very dense decorating of the DNA with the enzyme, which is important for the high sensitivity of the assay [citation needed]. The enzyme catalyzes a reaction of a substrate which ...
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