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Actinidain is active over a wide range of pH, including very acidic conditions, [20] with a pH optimum from 5-7. [21] At least ten different isoforms that all have the same molecular weight and cysteine protease activity as actinidain have been identified but they vary in isoelectric point from acidic (pI 3.9) to basic (pI 9.3). [19]
Caricain can be assayed with Bz-ArgkNHPhNO2, kcat/Km being 187 M21 s21 at pH 6.8 and 40˚C. More sensitive substrates may employ a fluorometric leaving group, kcat/Km for the hydrolysis of Z-Phe-ArgkNHMec being 1.06 3 106 M21 s21 (pH 6.8, 40˚C). [5] The enzyme exhibits a broad pH-activity profile, with the optimum near 7.0.
Experts agree that eating wholesome, healthy meals, reducing calories, and increasing physical activity are the best ways to lose weight and keep it off long-term. Still, many people turn to ...
In many flatworms, there are very high levels of expression of cysteine cathepsins; in the liver fluke Fasciola hepatica, gene duplications have produced over 20 paralogs of a cathepsin L-like enzyme. [1] Cysteine cathepsins are also part of the normal life cycle of the unicellular parasite Leishmania, where they function as virulence factors. [16]
Oxygen, hydrogen, carbon and nitrogen are the most abundant elements in the body by weight and make up about 96% of the weight of a human body. Calcium makes up 920 to 1200 grams of adult body weight, with 99% of it contained in bones and teeth. This is about 1.5% of body weight. [2]
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Phaseolus vulgaris, [40] bananas, chestnuts, kiwifruit, avocados, papaya, and tomatoes, for example, all contain significant levels of chitinase, as defense against fungal and invertebrate attack. Stress, or environmental signals like ethylene gas, may stimulate increased production of chitinase.
Coenzyme Q 10 (CoQ 10 / ˌ k oʊ k j uː ˈ t ɛ n /), also known as ubiquinone, is a naturally occurring biochemical cofactor (coenzyme) and an antioxidant produced by the human body. [1] [2] [3] It can also be obtained from dietary sources, such as meat, fish, seed oils, vegetables, and dietary supplements.
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