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Detection of viral RNA and DNA genomes can be performed using polymerase chain reaction. This technique makes many copies of the virus genome using virus-specific probes. Variations of PCR such as nested reverse transcriptase PCR and real time PCR can also be used to determine viral loads in patient serum.
Because ApMV can be asymptomatic for quite a while, it is also best to test for the pathogen using high sensitivity and high specificity detection methods such as ELISA. [1] Various other techniques have been utilized to eliminate the virus from diseased plant species. A common technique used in the past is thermotherapy. [1]
Traditional viral culture has been generally superseded by shell vial culture, in which the sample is centrifuged onto a single layer of cells and viral growth is measured by antigen detection methods. This greatly reduces the time to detection for slow growing viruses such as cytomegalovirus, for which the method was developed. [3]
Plant pathogens, organisms that cause infectious plant diseases, include fungi, oomycetes, bacteria, viruses, viroids, virus-like organisms, phytoplasmas, protozoa, nematodes and parasitic plants. [2] In most plant pathosystems, virulence depends on hydrolases and enzymes that degrade the cell wall.
Plant virus transmission strategies in insect vectors. Plant viruses need to be transmitted by a vector, most often insects such as leafhoppers. One class of viruses, the Rhabdoviridae, has been proposed to actually be insect viruses that have evolved to replicate in plants. The chosen insect vector of a plant virus will often be the ...
Gamma phage, an example of virus particles (visualised by electron microscopy) Virology is the scientific study of biological viruses.It is a subfield of microbiology that focuses on their detection, structure, classification and evolution, their methods of infection and exploitation of host cells for reproduction, their interaction with host organism physiology and immunity, the diseases they ...
Imaging-based approaches for the detection has two main methods, fluorescence imaging and hyper-spectral imaging. Fluorescence imaging helps identify the metabolic conditions of the plant. In order to do so, a tool is used to present light onto the chlorophyll complex of the plant. [7] Hyper-spectral imaging is used to obtain reflected images.
Cells infected by rotavirus (top) and uninfected cells (bottom). The focus forming assay (FFA) is a variation of the plaque assay, but instead of depending on cell lysis in order to detect plaque formation, the FFA employs immunostaining techniques using fluorescently labeled antibodies specific for a viral antigen to detect infected host cells and infectious virus particles before an actual ...