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The purpose of using RNA FISH is to detect target mRNA transcripts in cells, tissue sections, or even whole-mounts. [10] The process is done in 3 main procedures: tissue preparation (pre-hybridization), hybridization, and washing (post-hybridization). The tissue preparation starts by collecting the appropriate tissue sections to perform RNA FISH.
Probe design for CISH is very similar to that for FISH with differences only in labelling and detection. FISH probes are generally labelled with a variety of different fluorescent tags and can only be detected under a fluorescence microscope, [4] whereas CISH probes are labelled with biotin or digoxigenin [5] and can be detected using a bright-field microscope after other treatment steps have ...
Q-FISH. Quantitative Fluorescent in situ hybridization (Q-FISH) is a cytogenetic technique based on the traditional FISH methodology. In Q-FISH, the technique uses labelled (Cy3 or FITC) synthetic DNA mimics called peptide nucleic acid (PNA) oligonucleotides to quantify target sequences in chromosomal DNA using fluorescent microscopy and ...
Sample Ishikawa diagram shows the causes contributing to problem. The defect, or the problem to be solved, [1] is shown as the fish's head, facing to the right, with the causes extending to the left as fishbones; the ribs branch off the backbone for major causes, with sub-branches for root-causes, to as many levels as required.
This 16th-century fish stall shows many traditional fish products. The term fish processing refers to the processes associated with fish and fish products between the time fish are caught or harvested, and the time the final product is delivered to the customer. Although the term refers specifically to fish, in practice it is extended to cover ...
In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acid strand (i.e., a probe) to localize a specific DNA or RNA sequence in a portion or section of tissue (in situ) or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH ...
Pool-and-weir fish ladder at Bonneville Dam on the Columbia River Drone video of a fish way in Estonia, on the river Jägala FERC Fish Ladder Safety Sign. A fish ladder, also known as a fishway, fish pass, fish steps, or fish cannon, is a structure on or around artificial and natural barriers (such as dams, locks and waterfalls) to facilitate diadromous fishes' natural migration as well as ...
The first step of nitrification in the biofilter consumes alkalinity and lowers the pH of the system. [10] Keeping the pH in a suitable range (5.0-9.0 for freshwater systems) is crucial to maintain the health of both the fish and biofilter. pH is typically controlled by the addition of alkalinity in the form of lime (CaCO 3) or sodium hydroxide ...