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The nif genes are genes encoding enzymes involved in the fixation of atmospheric nitrogen into a form of nitrogen available to living organisms. The primary enzyme encoded by the nif genes is the nitrogenase complex which is in charge of converting atmospheric nitrogen (N 2) to other nitrogen forms such as ammonia which the organism can use for various purposes.
nifRLA operon: The tight expression regulation of the nitrogen fixation (nif) genes is mediated by the products of the nifRLA operon. NifA activates transcription of nif genes by the alternative form of RNA polymerase, s54-holoenzyme. NifL is a negative regulatory gene which inhibits the activation of other nif genes by nifA protein.
Human impact on the nitrogen cycle is diverse. Agricultural and industrial nitrogen (N) inputs to the environment currently exceed inputs from natural N fixation . [ 1 ] As a consequence of anthropogenic inputs, the global nitrogen cycle (Fig. 1) has been significantly altered over the past century.
A method for nitrogen fixation was first described by Henry Cavendish in 1784 using electric arcs reacting nitrogen and oxygen in air. This method was implemented in the Birkeland–Eyde process of 1903. [67] The fixation of nitrogen by lightning is a very similar natural occurring process.
Human activities such as fossil fuel combustion, use of artificial nitrogen fertilizers, and release of nitrogen in wastewater have dramatically altered the global nitrogen cycle. [17] [18] [19] Human modification of the global nitrogen cycle can negatively affect the natural environment system and also human health. [20] [21]
In the nitrogen cycle, atmospheric nitrogen is converted by plants into usable forms such as ammonia and nitrates through the process of nitrogen fixation. These compounds can be used by other organisms, and nitrogen is returned to the atmosphere through denitrification and other processes.
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Heterocysts or heterocytes are specialized nitrogen-fixing cells formed during nitrogen starvation by some filamentous cyanobacteria, such as Nostoc, Cylindrospermum, and Anabaena. [1] They fix nitrogen from dinitrogen (N 2) in the air using the enzyme nitrogenase, in order to provide the cells in the filament with nitrogen for biosynthesis. [2]