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Swinging head (or swinging bucket) centrifuges, in contrast to fixed-angle centrifuges, have a hinge where the sample containers are attached to the central rotor. This allows all of the samples to swing outwards as the centrifuge is spun. Continuous tubular centrifuges do not have individual sample vessels and are used for high volume ...
Centrifugation is the first step in most fractionations. Through low-speed centrifugation, cell debris may be removed, leaving a supernatant preserving the contents of the cell. Repeated centrifugation at progressively higher speeds will fractionate homogenates of cells into their components.
A laboratory centrifuge is a piece of laboratory equipment, driven by a motor, which spins liquid samples at high speed. There are various types of centrifuges, depending on the size and the sample capacity.
Svedberg won the Nobel Prize in Chemistry in 1926 for his research on colloids and proteins using the ultracentrifuge. [5] [6] [3] In early 1930s, Émile Henriot found that suitably placed jets of compressed air can spin a bearingless top to very high speeds and developed an ultracentrifuge on that principle.
Differential centrifugation, on the other hand, does not utilize a density gradient, and the centrifugation is taken in increasing speeds. The different centrifugation speeds often create separation into not more than two fractions, so the supernatant can be separated further in additional centrifugation steps.
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A tricanter centrifuge operates on a similar principle to decanter centrifuges but instead separates three phases, consisting of a suspended solids phase and two immiscible liquids. [5] Sedimentation of the suspended solids occurs as normal where they accumulate on the wall of the bowl and are conveyed out of the centrifuge.
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