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Mascot is a software search engine that uses mass spectrometry data to identify proteins from peptide sequence databases. [1] [2] Mascot is widely used by research facilities around the world.
UniProt is a freely accessible database of protein sequence and functional information, many entries being derived from genome sequencing projects.It contains a large amount of information about the biological function of proteins derived from the research literature.
The term "metabolome" was first used in 1998 [1] [4] and was likely coined to match with existing biological terms referring to the complete set of genes (the genome), the complete set of proteins (the proteome) and the complete set of transcripts (the transcriptome). The first book on metabolomics was published in 2003. [5]
Quantitative proteomics can circumvent these issues, only needing sequence information to be performed. It can be applied on a global proteome level, or on specifically isolating binding partners in pull-down or affinity purification experiments. [4] [22] Disadvantages, however, in sensitivity and analysis time must be kept in consideration. [23]
The total number of protein-coding genes (the Earth's proteome) is estimated to be 5 million sequences. [ 98 ] Although the number of base-pairs of DNA in the human genome has been known since the 1950s, the estimated number of genes has changed over time as definitions of genes, and methods of detecting them have been refined.
A mass spectrometer used for high throughput protein analysis. Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins.Mass spectrometry is an important method for the accurate mass determination and characterization of proteins, and a variety of methods and instrumentations have been developed for its many uses.
Proteostasis is the dynamic regulation of a balanced, functional proteome.The proteostasis network includes competing and integrated biological pathways within cells that control the biogenesis, folding, trafficking, and degradation of proteins present within and outside the cell.
The human cyclophilin family, as represented by the structures of the isomerase domains of some of its members. A protein family is a group of evolutionarily related proteins.In many cases, a protein family has a corresponding gene family, in which each gene encodes a corresponding protein with a 1:1 relationship.
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