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  2. Sticky and blunt ends - Wikipedia

    en.wikipedia.org/wiki/Sticky_and_blunt_ends

    The simplest DNA end of a double stranded molecule is called a blunt end. Blunt ends are also known as non-cohesive ends. In a blunt-ended molecule, both strands terminate in a base pair. Blunt ends are not always desired in biotechnology since when using a DNA ligase to join two molecules into one, the yield is significantly lower with blunt ...

  3. Ligation (molecular biology) - Wikipedia

    en.wikipedia.org/wiki/Ligation_(molecular_biology)

    PCR usually generates blunt-ended PCR products, but note that PCR using Taq polymerase can add an extra adenine (A) to the 3' end of the PCR product. This property may be exploited in TA cloning where the ends of the PCR product can anneal to the T end of a vector. TA ligation is therefore a form of sticky end ligation. Blunt-ended vectors may ...

  4. Lambda phage - Wikipedia

    en.wikipedia.org/wiki/Lambda_phage

    Enterobacteria phage λ (lambda phage, coliphage λ, officially Escherichia virus Lambda) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli (E. coli). It was discovered by Esther Lederberg in 1950. [ 2 ]

  5. Emboldened by ABC settlement, Trump threatens more lawsuits ...

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    For premium support please call: 800-290-4726 more ways to reach us. Sign in. Mail. 24/7 Help. For premium support please call: 800-290-4726 more ways to reach us. Mail. Sign in. Subscriptions ...

  6. The end-call button on your iPhone could move soon. What to ...

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    The location of Apple's red end-call button is set to slightly move with upcoming iOS 17 updates to the phone app, so be wary of your thumb's muscle memory. As iPhone users know, the “End ...

  7. Cohesive end - Wikipedia

    en.wikipedia.org/?title=Cohesive_end&redirect=no

    Retrieved from "https://en.wikipedia.org/w/index.php?title=Cohesive_end&oldid=476062181"This page was last edited on 10 February 2012, at 03:38 (UTC). (UTC).

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  9. Mobile genetic elements - Wikipedia

    en.wikipedia.org/wiki/Mobile_genetic_elements

    Simultaneously the donor strand is ligated to the target strand after cleavage leaving a single strand overhang on either end of the target sequence. These sites usually contain a 5 to 9 base pair overhang that can create a cohesive end. [10] Transposase then holds the sequence in a crossed formation and ligates the donor strand to the target ...