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  2. RNA-Seq - Wikipedia

    en.wikipedia.org/wiki/RNA-Seq

    Long-read sequencing captures the full transcript and thus minimizes many of issues in estimating isoform abundance, like ambiguous read mapping. For short-read RNA-Seq, there are multiple methods to detect alternative splicing that can be classified into three main groups: [119] [91] [120]

  3. 3' mRNA-seq - Wikipedia

    en.wikipedia.org/wiki/3'_mRNA-seq

    3' mRNA-seq methods are generally cheaper per sample than standard bulk RNA-seq methods. [2] [7] [8] [9] This is because of the lower sequencing depth required due to only the 3' end of mRNA molecules being sequenced instead of the whole length of entire transcripts. Read depths of between one million and five million reads are recommended in ...

  4. BRB-seq - Wikipedia

    en.wikipedia.org/wiki/BRB-seq

    Schematic overview of the MERCURIUS BRB-seq workflow where up to 384 samples can be barcoded and multiplexed per kit.. Bulk RNA barcoding and sequencing (BRB-seq) is an ultra-high-throughput bulk 3' mRNA-seq technology that uses early-stage sample barcoding and unique molecular identifiers (UMIs) to allow the pooling of up to 384 samples in one tube early in the sequencing library preparation ...

  5. Single-cell transcriptomics - Wikipedia

    en.wikipedia.org/wiki/Single-cell_transcriptomics

    RNA Seq Experiment. The single-cell RNA-seq technique converts a population of RNAs to a library of cDNA fragments. These fragments are sequenced by high-throughput next generation sequencing techniques and the reads are mapped back to the reference genome, providing a count of the number of reads associated with each gene. [13]

  6. Transcriptomics technologies - Wikipedia

    en.wikipedia.org/wiki/Transcriptomics_technologies

    RNA-Seq methodology has constantly improved, primarily through the development of DNA sequencing technologies to increase throughput, accuracy, and read length. [61] Since the first descriptions in 2006 and 2008, [ 40 ] [ 62 ] RNA-Seq has been rapidly adopted and overtook microarrays as the dominant transcriptomics technique in 2015.

  7. Time-resolved RNA sequencing - Wikipedia

    en.wikipedia.org/wiki/Time-resolved_RNA_sequencing

    Time-resolved RNA sequencing methods are applications of RNA-seq that allow for observations of RNA abundances over time in a biological sample or samples. Second-Generation DNA sequencing has enabled cost effective, high throughput and unbiased analysis of the transcriptome. [1] Normally, RNA-seq is only capable of capturing a snapshot of the ...

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    The search engine that helps you find exactly what you're looking for. Find the most relevant information, video, images, and answers from all across the Web.

  9. Read (biology) - Wikipedia

    en.wikipedia.org/wiki/Read_(biology)

    Sequencing technologies vary in the length of reads produced. Reads of length 20-40 base pairs (bp) are referred to as ultra-short. [2] Typical sequencers produce read lengths in the range of 100-500 bp. [3] However, Pacific Biosciences platforms produce read lengths of approximately 1500 bp. [4] Read length is a factor which can affect the results of biological studies. [5]