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Dische's diphenylamine reagent consists of diphenylamine, glacial acetic acid, sulfuric acid, and ethanol. [1] When heated with DNA, it turns blue in the presence of DNA. A more intense blue color indicates a greater concentration of DNA.
Diphenylamine is an organic compound with the formula (C 6 H 5) 2 NH. The compound is a derivative of aniline , consisting of an amine bound to two phenyl groups. The compound is a colorless solid, but commercial samples are often yellow due to oxidized impurities. [ 5 ]
Zacharias Dische (18 February 1895 – 17 January 1988) was an American biochemist. He worked as a biochemical researcher in Vienna before being forced by the Anschluss to become a refugee, [ 1 ] first in France and then in the US, where he joined the faculty of Columbia University in 1943. [ 2 ]
The polymerase chain reaction is the most widely used method for in vitro DNA amplification for purposes of molecular biology and biomedical research. [1] This process involves the separation of the double-stranded DNA in high heat into single strands (the denaturation step, typically achieved at 95–97 °C), annealing of the primers to the single stranded DNA (the annealing step) and copying ...
More than 100,000 backlogged sexual assault kits have been sent for testing over the past decade, many with funding from a federal grant program known as the National Sexual Assault Kit Initiative.
Genetic testing, also known as DNA testing, is used to identify changes in DNA sequence or chromosome structure. Genetic testing can also include measuring the results of genetic changes, such as RNA analysis as an output of gene expression , or through biochemical analysis to measure specific protein output. [ 1 ]
PLYMOUTH − The attorney for Lindsay Clancy, the Duxbury woman facing murder charges in the deaths of her three children, has agreed to prosecutors' request to test her DNA.. During the first ...
The Riverside County Sheriff’s Office used DNA and forensic genealogy to identify the suspected killer, who turned out to be the same man who reported finding Esther Gonzalez’s body to ...
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