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Simple computations in all individuals (i.e. fish) Various means of storing information (i.e. weights of fish and school barycenter) Local computations (i.e. swimming is composed of distinct components) Low communications between neighboring individuals (i.e. fish are to think local but also be socially aware)
Probe design for CISH is very similar to that for FISH with differences only in labelling and detection. FISH probes are generally labelled with a variety of different fluorescent tags and can only be detected under a fluorescence microscope, [4] whereas CISH probes are labelled with biotin or digoxigenin [5] and can be detected using a bright-field microscope after other treatment steps have ...
Quantitative Fluorescent in situ hybridization (Q-FISH) is a cytogenetic technique based on the traditional FISH methodology. In Q-FISH, the technique uses labelled (Cy3 or FITC) synthetic DNA mimics called peptide nucleic acid (PNA) oligonucleotides to quantify target sequences in chromosomal DNA using fluorescent microscopy and analysis software.
Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only particular parts of a nucleic acid sequence with a high degree of sequence complementarity.
Nick translation [1] (or head translation), developed in 1977 by Peter Rigby and Paul Berg, is a tagging technique in molecular biology in which DNA polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which can be used as a probe in fluorescent in situ hybridization (FISH) or blotting techniques.
The technique, in its most simple iteration, consists in setting up net barriers to trap the tuna when they migrate into the Mediterranean Sea from the Atlantic Ocean (February to July), on their way to spawn and until recently, on their return journey, ("al revés"); the bycatch contains, among others, bullet tuna (auxis rochei), little tunny ...