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A UV-Vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet (UV) and visible light that is absorbed by a sample. It is a widely used technique in chemistry, biochemistry, and other fields, to identify and quantify compounds in a variety of samples.
Spectrophotometry is a tool that hinges on the quantitative analysis of molecules depending on how much light is absorbed by colored compounds. Important features of spectrophotometers are spectral bandwidth (the range of colors it can transmit through the test sample), the percentage of sample transmission, the logarithmic range of sample ...
Jablonski diagram including vibrational levels for absorbance, non-radiative decay, and fluorescence.. When a molecule absorbs a photon, the photon energy is converted and increases the molecule's internal energy level.
Using the Bausch & Lomb Spectronic 20 Colorimeter, 1962. The Spectronic 20 is a brand of single-beam spectrophotometer, designed to operate in the visible spectrum [1] across a wavelength range of 340 nm to 950 nm, with a spectral bandpass of 20 nm.
The Soret band arises primarily due to an electron dipole movement that allows π-π* transitions; most common in porphyrin compounds. [2] Most analytical studies of porphyrin‑containing moiety can be done using ultraviolet–visible spectroscopy and exciting wavelength at the respective Soret band wavelength.
The goal of absorption spectroscopy techniques (FTIR, ultraviolet-visible ("UV-vis") spectroscopy, etc.) is to measure how much light a sample absorbs at each wavelength. [2] The most straightforward way to do this, the "dispersive spectroscopy" technique, is to shine a monochromatic light beam at a sample, measure how much of the light is ...
Photomultiplier Handbook (4MB PDF) from Burle Industries, essentially the Engstrom-RCA Handbook reprinted; Photomultiplier technical papers from ET-Enterprises; Photomultiplier tubes basics and applications from Hamamatsu Photonics; Electron Multiplier – simulation of an electron multiplier tube
For UV-Vis spectroelectrochemistry, spectrometer must be specific for UV-Vis spectral region. Potentiostat / Galvanostat : electronic device that allows controlling the working electrode potential regarding to the reference electrode or controlling the current that passes respect to the auxiliary electrode .