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  2. Escherichia coli BL21(DE3) - Wikipedia

    en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3)

    The LacUV5 promoter is derived from the E. coli wildtype lac promoter but exhibits an increased transcription strength due to two mutations that facilitate its interaction with a native E. coli RNAP σ-factor. [7] In E. coli BL21(DE3) the expression of the T7-RNAP is suppressed by the constitutively expressed LacI repressor.

  3. Lactase - Wikipedia

    en.wikipedia.org/wiki/Lactase

    Studies of E. coli lactase have proposed that hydrolysis is initiated when a glutamate nucleophile on the enzyme attacks from the axial side of the galactosyl carbon in the β-glycosidic bond. [16] The removal of the D -glucose leaving group may be facilitated by Mg-dependent acid catalysis. [ 16 ]

  4. Coliform bacteria - Wikipedia

    en.wikipedia.org/wiki/Coliform_bacteria

    Escherichia coli (E. coli) can be distinguished from most other coliforms by its ability to ferment lactose at 44 °C in the fecal coliform test, and by its growth and color reaction on certain types of culture media. When cultured on an eosin methylene blue (EMB) plate, a positive result for E. coli is metallic green colonies on a dark purple ...

  5. What to know about deadly McDonald's E. coli outbreak

    www.aol.com/1-dead-49-sickened-e-212614030.html

    What to know about E. coli symptoms, recovery. Although most E. coli bacteria are harmless and are part of a healthy intestinal tract, some strains of the bacteria can make people sick, according ...

  6. Coliform index - Wikipedia

    en.wikipedia.org/wiki/Coliform_index

    By testing for coliforms, especially the well known Escherichia coli (E. coli), which is a thermotolerant coliform, one can determine if the water has possibly been exposed to fecal contamination; that is, whether it has come in contact with human or animal feces. It is important to know this because many disease-causing organisms are ...

  7. TSI slant - Wikipedia

    en.wikipedia.org/wiki/TSI_slant

    TSI agar slant results: (from left) preinoculated (as control), P. aeruginosa, E. coli, Salmonella Typhimurium, Shigella flexneri The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. [1]

  8. E. coli infections tied to McDonald's burgers rise to 75, US ...

    www.aol.com/news/us-fda-says-75-people-151321897...

    The E. coli O157:H7 strain that has caused the death of one person is said to cause "very serious disease", especially for the elderly, children and people who are immunocompromised.

  9. Fecal coliform - Wikipedia

    en.wikipedia.org/wiki/Fecal_coliform

    The fecal coliform assay is intended to be an indicator of fecal contamination; more specifically of E. coli which is an indicator microorganism for other pathogens that may be present in feces. Presence of fecal coliforms in water may not be directly harmful, and do not necessarily indicate the presence of feces.

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