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In biochemistry, denaturation is a process in which proteins or nucleic acids lose folded structure present in their native state due to various factors, including application of some external stress or compound, such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), agitation and radiation, or heat. [3]
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Denaturation Mapping is a form of optical mapping, first described in 1966. It is used to characterize DNA molecules without the need for amplification or sequencing . It is based on the differences between the melting temperatures of AT-rich and GC-rich regions. [ 1 ]
The most famous example is the hyperchromicity of DNA that occurs when the DNA duplex is denatured. [1] The UV absorption is increased when the two single DNA strands are being separated, either by heat or by addition of denaturant or by increasing the pH level. The opposite, a decrease of absorbance is called hypochromicity.
Biochemistry and Cell Biology; Biochimica et Biophysica Acta; Biotechnology and Applied Biochemistry; Cell Biochemistry & Function; Comparative Biochemistry and Physiology B; Critical Reviews in Biochemistry and Molecular Biology; FEBS Journal; FEBS Letters; Journal of Biochemistry; Journal of Biological Chemistry; Journal of Liposome Research
Biology: Open access preprint repository for the biological sciences >100,000 [9] 2013 Cold Spring Harbor Laboratory: BodoArXiv: Medieval Studies: Open Repository for Medieval Studies <100 2019 ScholarlyHub & Center for Open Science: Cell Sneak Peek: Biology: Manuscript under review in Cell Press journals >1,000 2018 Elsevier: ChemRxiv [10 ...
Guanidinium thiocyanate can be used to deactivate a virus, such as the influenza virus that caused the 1918 "Spanish flu", so that it can be studied safely.. Guanidinium thiocyanate is also used to lyse cells and virus particles in RNA and DNA extractions, where its function, in addition to its lysing action, is to prevent activity of RNase enzymes and DNase enzymes by denaturing them.
This is a list of open-access journals by field. The list contains notable journals which have a policy of full open access. It does not include delayed open access journals, hybrid open access journals, or related collections or indexing services. True open-access journals can be split into two categories: