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3-Amino-9-ethylcarbazole (AEC) is a chemical compound commonly used as a chromogenic substrate in immunohistochemistry, specifically for visualizing sections stained ...
Some examples of substrates used (also known as chromogens) are AEC (3-Amino-9-EthylCarbazole), or DAB (3,3'-Diaminobenzidine). Use of one of these reagents after exposure to the necessary enzyme (e.g., horseradish peroxidase conjugated to an antibody reagent) produces a positive immunoreaction product.
3-Amino-9-ethylcarbazole (AEC) Naphazoline This page was last edited on 26 August 2022, at 20:01 (UTC). Text is available under the Creative Commons Attribution ...
3-amino-9-ethylcarbazole, an immunohistochemistry stain (chromogen) Abstract elementary class, in mathematical logic; Acoustic echo cancellation, in telephony; Automatic exposure control, in radiography; Anion exchange capacity, in pedology (soil sciences)
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It is prepared by condensation of chloranil and 3-amino-N-ethylcarbazole. It has a centrosymmetric angular structure. [ 2 ] For many years, the structure was assigned, incorrectly, as having a "linear structure" ( EC no. 228-767-9, CAS RN 6358-30-1) which differ in terms of the carbazole ring fusion .
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In the first step, an N-phenyl-1,2-diaminobenzene (N-phenyl-o-phenylenediamine) is converted into a diazonium salt which instantaneously forms a 1,2,3-triazole. The triazole is unstable and at elevated temperatures, nitrogen is released and the carbazole is formed. [11] [12] Fluorescence of (9H-carbazol-9-yl)(2,4-dichlorophenyl) methanone