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Paper chromatography is an analytical method used to separate colored chemicals or substances. It can also be used for colorless chemicals that can be located by a stain or other visualisation method after separation. [ 1 ]
Chromatography is a method chemists use to separate compounds. This type of filter paper has specific water flow rate and absorption speed to maximize the result of paper chromatography. The absorption speed of this type of filter paper is from 6 cm to 18 cm and the thickness is from 0.17 mm from 0.93 mm. [9]
Planar chromatography is a separation technique in which the stationary phase is present as or on a plane. The plane can be a paper, serving as such or impregnated by a substance as the stationary bed (paper chromatography) or a layer of solid particles spread on a support such as a glass plate (thin-layer chromatography).
Mr. Whatman's Turkey Paper Mills by Paul Sandby (1794) The papermaker James Whatman the Elder (1702–1759) founded the Whatman papermaking enterprise in 1740 in Maidstone , Kent , England. He made revolutionary advances to the craft in England and is credited [ 2 ] as the inventor of wove paper (or Vélin), an innovation used for high-quality ...
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The introduction of paper chromatography was an important analytical technique which gave rise to thin-layer chromatography. [13] Finally, gas-liquid chromatography, a fundamental technique in modern analytical chemistry, was described by Martin with coauthors A. T. James and G. Howard Smith in 1952.
Finally, the HPTLC plate is placed in the chamber to develop. Between each sample reading, the mobile phase and filter paper are changed to ensure the best outcomes. The spot capacity (analogous to peak capacity in HPLC) can be increased by developing the plate with two different solvents, using two-dimensional chromatography. [8]
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