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Buffer capacity falls to 33% of the maximum value at pH = pK a ± 1, to 10% at pH = pK a ± 1.5 and to 1% at pH = pK a ± 2. For this reason the most useful range is approximately pK a ± 1. When choosing a buffer for use at a specific pH, it should have a pK a value as close as possible to that pH. [2]
The following table presents pK a values at 20 °C. Values change by about 0.01 per degree of temperature. [1] [3] Good's original 1966 paper had two older buffers (marked with italics) for comparison.
It consists of a mixture of 0.04 M boric acid, 0.04 M phosphoric acid and 0.04 M acetic acid that has been titrated to the desired pH with 0.2 M sodium hydroxide. Britton and Robinson also proposed a second formulation that gave an essentially linear pH response to added alkali from pH 2.5 to pH 9.2 (and buffers to pH 12).
The following is a sample recipe for TBST: 20 mM Tris; 150 mM NaCl; 0.1% Tween 20; Adjust pH with HCl to pH 7.4–7.6 The simplest way to prepare a TBS-Tween solution is to use TBS-T tablets.
TE buffer is also known as T 10 E 1 buffer, which can be read as "T ten E one buffer". To make a 100 ml solution of T 10 E 1 buffer, 1 ml of 1 M Tris base (pH 10–11) and 0.2 ml EDTA (0.5 M) are mixed and made up with double distilled water up to 100ml. Add microliter amounts of high molarity HCl to lower the pH to 8. Based on nuclease studies ...
Adjust pH to pH 8.2 The simplest way to prepare a BBS solution is to use BBS tablets. They are formulated to give a ready to use borate buffered saline solution upon dissolution in 500 ml of deionized water. Concentration of borate and NaCl as well as the pH can vary, and the resulting solution would still be referred to as "borate buffered ...
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