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An optical instrument is a device that processes light waves (or photons), either to enhance an image for viewing or to analyze and determine their characteristic properties. Common examples include periscopes, microscopes, telescopes, and cameras. [1] [2]
The wavelength of visible light waves varies between 400 and 700 nm, but the term "light" is also often applied to infrared (0.7–300 μm) and ultraviolet radiation (10–400 nm). The wave model can be used to make predictions about how an optical system will behave without requiring an explanation of what is "waving" in what medium.
Visible light (and near-infrared light) is typically absorbed and emitted by electrons in molecules and atoms that move from one energy level to another. This action allows the chemical mechanisms that underlie human vision and plant photosynthesis. The light that excites the human visual system is a very small portion of the electromagnetic ...
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
By recording the attenuation of light for various wavelengths, an absorption spectrum can be obtained. In physics , absorption of electromagnetic radiation is how matter (typically electrons bound in atoms ) takes up a photon 's energy —and so transforms electromagnetic energy into internal energy of the absorber (for example, thermal energy ).
A two-photon microscope is also a laser-scanning microscope, but instead of UV, blue or green laser light, a pulsed infrared laser is used for excitation. Only in the tiny focus of the laser is the intensity high enough to generate fluorescence by two-photon excitation , which means that no out-of-focus fluorescence is generated, and no pinhole ...
The dispersion of light by glass prisms is used to construct spectrometers and spectroradiometers. However, in lenses, dispersion causes chromatic aberration, an undesired effect that may degrade images in microscopes, telescopes, and photographic objectives. The phase velocity v of a wave in a given uniform medium is given by
Without oil, light waves reflect off the slide specimen through the glass cover slip, through the air, and into the microscope lens (see the colored figure to the right). Unless a wave comes out at a 90-degree angle, it bends when it hits a new substance, the amount of bend depending on the angle. This distorts the image.
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