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These aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain. • PAS diastase stain (PAS-D) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen. • Alcian blue/periodic acid–Schiff (AB/PAS or AB-PAS) uses alcian blue before the PAS step.
In its use as a qualitative test for aldehydes, the unknown sample is added to the decolorized Schiff reagent; when aldehyde is present a characteristic magenta color develops. Schiff-type reagents are used for various biological tissue staining methods, e.g. Feulgen stain and periodic acid-Schiff stain.
Oxidize in Periodic Acid, 0.5%, 5 minutes and rinse in distilled water; Strain in Coleman's Feulgen, or Schiff's Reagent, 15 minutes and wash in running water to develop the pink color, 10 minutes. Counter stain in Haris Hematoxylin, 6 minutes, or Light Green working Solution, 10 seconds.
Periodic acid–Schiff–diastase (PAS-D, PAS diastase) stain is a periodic acid–Schiff (PAS) stain used in combination with diastase, an enzyme that breaks down glycogen. PAS-D is a stain often used by pathologists as an ancillary study in making a histologic diagnosis on paraffin -embedded tissue specimens.
Smear is treated for hydrolysis to release purines from DNA, purines to cause shift form furanose to aldehyde. Aldehyde groups are available to react with schiff's reagent to form addition compounds. To demonstrate the presence of DNA in cell. But for detection of the DNA, RNA should be selectively destroyed by acid hydrolysis without affecting DNA
Sanger reagent; Saponification; Sarett oxidation; Schiemann reaction [18] Schiff reaction; Schiff test; Schlenk equilibrium; Schlosser modification; Schlosser variant; Schmidlin ketene synthesis; Schmidt degradation; Schmidt reaction; Scholl reaction; Schorigin Shorygin reaction, Shorygin reaction, Wanklyn reaction; Schotten–Baumann reaction ...
Jones' stain, also Jones stain, is a methenamine silver–periodic acid–Schiff stain used in pathology. [1] It is also referred to as methenamine PAS which is commonly abbreviated MPAS. It stains for basement membrane and is widely used in the investigation of medical kidney diseases.
After separation of red cell membranes by SDS-polyacrylamide gel electrophoresis and staining with periodic acid-Schiff staining (PAS), four glycophorins have been identified. These have been named glycophorin A, B, C, and D in order of the quantity present in the membrane, glycophorin A being the most and glycophorin D the least common.