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Zymography is an electrophoretic technique for the detection of hydrolytic enzymes, based on the substrate repertoire of the enzyme. Three types of zymography are used; in gel zymography, in situ zymography and in vivo zymography. [2] For instance, gelatin embedded in a polyacrylamide gel will be digested by active gelatinases run through the gel.
Cathepsin zymography is a technique for quantifying enzymatic activity of the cathepsin family of cysteine proteases. It is based on SDS-PAGE whereby samples tested for cathepsin activity are loaded into a polyacrylamide gel and then separated by molecular weight.
In situ [a] is a Latin phrase meaning "in place" or "on site", derived from in ('in') and situ (ablative of situs, lit. ' place ' ). [ 3 ] The term refers to the examination, occurrence, or execution of a process within its original or natural context, without relocation.
In situ hybridization; In vitro; Indirect immunoperoxidase assay; Induced cell cycle arrest; Inert salt; Intravital microscopy; Inverse polymerase chain reaction; Iodine–starch test; Ion-mobility spectrometry–mass spectrometry; Ion-exchange membrane; Isotope dilution; Isotopic labeling
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Two Indian chemical companies have been indicted for allegedly importing ingredients for the highly addictive opioid fentanyl into the United States and Mexico, the U.S. Department of Justice said ...
A painting bought at a garage sale in Minnesota is a previously unknown portrait by Dutch artist Vincent Van Gogh, according to a newly published expert analysis.. It was made by Van Gogh during ...