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Second-generation sequencing has yielded many draft genomes (close to 90% of bacterial genomes in GenBank are currently not complete); third-generation sequencing might eventually yield a complete genome in a few hours. The genome sequences reveal much diversity in bacteria.
This list of sequenced eubacterial genomes contains most of the eubacteria known to have publicly available complete genome sequences.Most of these sequences have been placed in the International Nucleotide Sequence Database Collaboration, a public database which can be searched [1] on the web.
The first bacterial whole genome to be sequenced was of the bacterium Haemophilus influenzae. The worm Caenorhabditis elegans was the first animal to have its whole genome sequenced. Drosophila melanogaster 's whole genome was sequenced in 2000. Arabidopsis thaliana was the first plant genome sequenced.
BASys (Bacterial Annotation System) is a freely available web server that can be used to perform automated, comprehensive annotation of bacterial genomes. [2] With the advent of next generation DNA sequencing it is now possible to sequence the complete genome of a bacterium (typically ~4 million bases) within a single day.
All of the genome annotations in BacMap were generated through the BASys genome annotation system. [3] BASys is a widely used microbial annotation infrastructure that performs comprehensive bioniformatic analyses on raw (or labeled) bacterial genome sequence data.
The approach, used to sequence many cultured microorganisms and the human genome, randomly shears DNA, sequences many short sequences, and reconstructs them into a consensus sequence. Shotgun sequencing reveals genes present in environmental samples. Historically, clone libraries were used to facilitate this sequencing.
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