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  2. Spin column-based nucleic acid purification - Wikipedia

    en.wikipedia.org/wiki/Spin_column-based_nucleic...

    The different stages of the method are lyse, bind, wash, and elute. [1] [2] More specifically, this entails the lysis of target cells to release nucleic acids, selective binding of nucleic acid to a silica membrane, washing away particulates and inhibitors that are not bound to the silica membrane, and elution of the nucleic acid, with the end result being purified nucleic acid in an aqueous ...

  3. Gel extraction - Wikipedia

    en.wikipedia.org/wiki/Gel_extraction

    Gel extraction kits are available from several major biotech manufacturers for a final cost of approximately 1–2 US$ per sample. Protocols included in these kits generally call for the dissolution of the gel-slice in 3 volumes of chaotropic agent at 50 °C, followed by application of the solution to a spin-column (the DNA remains in the column), a 70% ethanol wash (the DNA remains in the ...

  4. Guanidinium thiocyanate - Wikipedia

    en.wikipedia.org/wiki/Guanidinium_thiocyanate

    Guanidinium thiocyanate can be used to deactivate a virus, such as the influenza virus that caused the 1918 "Spanish flu", so that it can be studied safely.. Guanidinium thiocyanate is also used to lyse cells and virus particles in RNA and DNA extractions, where its function, in addition to its lysing action, is to prevent activity of RNase enzymes and DNase enzymes by denaturing them.

  5. Plasmid preparation - Wikipedia

    en.wikipedia.org/wiki/Plasmid_preparation

    Plasmid miniprep. 0.8% agarose gel ethidium bromide-stained.. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA.It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology.

  6. DNA separation by silica adsorption - Wikipedia

    en.wikipedia.org/wiki/DNA_separation_by_silica...

    A further explanation of how DNA binds to silica is based on the action of guanidinium chloride (GuHCl), which acts as a chaotrope. [3] A chaotrope denatures biomolecules by disrupting the shell of hydration around them.

  7. Tom Kim apologizes for breaking locker door after playoff ...

    www.aol.com/sports/tom-kim-apologizes-breaking...

    Tom Kim apologized on social media Monday after he damaged his locker room door following a playoff loss at the DP World Tour’s Genesis Championship in South Korea on Sunday.

  8. When do the second College Football Playoff rankings come out?

    www.aol.com/second-college-football-playoff...

    Ranking 5: Tuesday, Dec. 3 at 7 p.m. - 7:30 p.m. Selection Day: Sunday, Dec. 8 at 12 p.m. -4 p.m. We occasionally recommend interesting products and services. If you make a purchase by clicking ...

  9. Gel electrophoresis - Wikipedia

    en.wikipedia.org/wiki/Gel_electrophoresis

    Gels suppress the thermal convection caused by the application of the electric field, and can also act as a sieving medium, slowing the passage of molecules; gels can also simply serve to maintain the finished separation so that a post electrophoresis stain can be applied. [3] DNA gel electrophoresis is usually performed for analytical purposes ...