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Activator binds to an inducer and the complex binds to the activation sequence and activates target gene. [2] Removing the inducer stops transcription. [2] Because a small inducer molecule is required, the increased expression of the target gene is called induction. [2] The lactose operon is one example of an inducible system. [2]
An inducer is the axial inlet portion of a centrifugal pump rotor, the function of which is to raise the inlet head by an amount sufficient to prevent significant cavitation in the following pump stage. [1] It is used in applications in which the inlet pressure of a pump is close to the vapor pressure of the pumped liquid.
[1] [2] [3] Activator-binding sites may be located very close to the promoter or numerous base pairs away. [2] [3] If the regulatory sequence is located far away, the DNA will loop over itself (DNA looping) in order for the bound activator to interact with the transcription machinery at the promoter site. [2] [3]
Toggle switch which operates using two mutually inhibitory genes, each promoter is inhibited by the repressor that is transcribed by the opposing promoter. Toggle switch design: Inducer 1 inactivates repressor 1, which means repressor 2 is produced. Repressor 2, in turn, stops transcription of the repressor 1 gene and the reporter gene. [14]
As an example, consider the use of a 10 hp, 1760 r/min, 440 V, three-phase induction motor (a.k.a. induction electrical machine in an asynchronous generator regime) as asynchronous generator. The full-load current of the motor is 10 A and the full-load power factor is 0.8. Required capacitance per phase if capacitors are connected in delta:
A typical operon. In genetics, an operon is a functioning unit of DNA containing a cluster of genes under the control of a single promoter. [1] The genes are transcribed together into an mRNA strand and either translated together in the cytoplasm, or undergo splicing to create monocistronic mRNAs that are translated separately, i.e. several strands of mRNA that each encode a single gene product.
When stored as a powder at 4 °C or below, IPTG is stable for 5 years. It is significantly less stable in solution; Sigma recommends storage for no more than a month at room temperature. [3] IPTG is an effective inducer of protein expression in the concentration range of 100 μmol/L to 3.0 mmol/L. Typically, a sterile, filtered 1 mol/L solution ...
Catabolite activator protein (CAP; also known as cAMP receptor protein, CRP) is a trans-acting transcriptional activator that exists as a homodimer in solution. Each subunit of CAP is composed of a ligand -binding domain at the N-terminus (CAP N , residues 1–138) and a DNA-binding domain at the C-terminus (DBD, residues 139–209).