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  2. Polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction

    A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.

  3. Real-time polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Real-time_polymerase_chain...

    A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used ...

  4. Reverse transcription polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Reverse_transcription...

    RT-PCR. Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR). [1] It is primarily used to measure the amount of a specific RNA.

  5. Principal component regression - Wikipedia

    en.wikipedia.org/wiki/Principal_component_regression

    In statistics, principal component regression (PCR) is a regression analysis technique that is based on principal component analysis (PCA). PCR is a form of reduced rank regression. [1] More specifically, PCR is used for estimating the unknown regression coefficients in a standard linear regression model.

  6. PCR - Wikipedia

    en.wikipedia.org/wiki/PCR

    Pathologic complete response (pCR), in neoadjuvant therapy; Polymerase chain reaction. COVID-19 testing, often performed using the polymerase chain reaction method; Phosphocreatine, a phosphorylated creatine molecule; Principal component regression, a statistical technique; Protein/creatinine ratio, in urine

  7. DNA profiling - Wikipedia

    en.wikipedia.org/wiki/DNA_profiling

    This technique was developed in 1983 by Kary Mullis. PCR is now a common and important technique used in medical and biological research labs for a variety of applications. [19] PCR, or Polymerase Chain Reaction, is a widely used molecular biology technique to amplify a specific DNA sequence. steps of polymerase chain reaction

  8. Variants of PCR - Wikipedia

    en.wikipedia.org/wiki/Variants_of_PCR

    Analysis of the genotypes in the samples usually involves sizing of the amplification products by gel electrophoresis. Analysis of smaller VNTR segments known as short tandem repeats (or STRs) is the basis for DNA fingerprinting databases such as CODIS. Asymmetric PCR preferentially

  9. Amplicon - Wikipedia

    en.wikipedia.org/wiki/Amplicon

    Analysis of amplicons has been made possible by the development of amplification methods such as PCR, and increasingly by cheaper and more high-throughput technologies for DNA sequencing or next-generation sequencing, such as ion semiconductor sequencing, popularly referred to as the brand of the developer, Ion Torrent.

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