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Giemsa stained Trypanosoma parasites (Chagas disease pathogen) Whirling disease section stained with Giemsa stain. Giemsa stain (/ ˈ ɡ iː m z ə /), named after German chemist and bacteriologist Gustav Giemsa, is a nucleic acid stain used in cytogenetics and for the histopathological diagnosis of malaria and other parasites.
Brugia is a genus for a group of small roundworms. They are among roundworms that cause the parasitic disease filariasis. [1] Specifically, of the three species known, Brugia malayi and Brugia timori cause lymphatic filariasis in humans; and Brugia pahangi and Brugia patei infect domestic cats, dogs and other animals.
Filariasis is usually diagnosed by identifying microfilariae on Giemsa stained, thin and thick blood film smears, using the "gold standard" known as the finger prick test. The finger prick test draws blood from the capillaries of the finger tip; larger veins can be used for blood extraction, but strict windows of the time of day must be observed.
The blood sample can be a thick smear, stained with Giemsa or haematoxylin and eosin. For increased sensitivity , concentration techniques can be used. These include centrifugation of the blood sample lyzed in 2% formalin (Knott's technique), or filtration through a nucleopore membrane .
Leishmania major is a species of parasite found in the genus Leishmania, and is associated with the disease zoonotic cutaneous leishmaniasis (also known as Aleppo boil, Baghdad boil, Bay sore, Biskra button, Chiclero ulcer, Delhi boil, Kandahar sore, Lahore sore, Oriental sore, Pian bois, and Uta). [1]
Dogs that have been exposed can take up to seven days to exhibit symptoms, including: lethargy. fever. vomiting. diarrhea. enlarged lymph nodes. Many dogs infected with the disease will need ...
Whole blood with microfilaria worm, giemsa stain. L. loa worms have a simple structure consisting of a head (which lacks lips), a body, and a blunt tail. The outer body of the worm is composed of a cuticle with three main layers made up of collagen and other compounds which aid in protecting the nematodes while they are inside the digestive system of their host.
The definitive diagnostic test is the identification of parasites on a Giemsa-stained thin-film blood smear. [ 13 ] So-called "Maltese cross formations" on the blood film are diagnostic ( pathognomonic ) of babesiosis, since they are not seen in malaria, the primary differential diagnosis. [ 11 ]
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