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Typhidot is a medical test consisting of a dot ELISA kit [1] that detects IgM and IgG antibodies against the outer membrane protein (OMP) of the Salmonella typhi.The typhidot test becomes positive within 2–3 days of infection and separately identifies IgM and IgG antibodies.
False positive and false negative results may occur. Test results need to be interpreted carefully to account for any history of enteric fever, typhoid vaccination, and the general level of antibodies in the populations in endemic areas of the world. As with all serological tests, the rise in antibody levels needed to perform the diagnosis ...
The sample pad of this kit contains colloidal gold-anti-human IgG or gold-anti-human IgM. If the sample contains IgG and IgM antibodies against those antigens, they will react and turn red. The typhidot test becomes positive within 2–3 days of infection. [citation needed] Two colored bands indicate a positive test.
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The complement system is a system of serum proteins that react with antigen-antibody complexes. If this reaction occurs on a cell surface, it will result in the formation of trans-membrane pores and therefore destruction of the cell. The basic steps of a complement fixation test are as follows: [1] Serum is separated from the patient.
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As a result, it has largely been supplanted by other methods of serology, including indirect immunofluorescence antibody (IFA) testing, which is the gold standard. However, in resource-limited settings, it still remains an important tool in the diagnosis and identification of public health concerns, such as outbreaks of epidemic typhus.