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ortho-Nitrophenyl-β-galactoside (ONPG) is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity. [1] This compound is normally colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol .
The term "IMViC" is an acronym for each of these tests. "I" is for indole test; "M" is for methyl red test; "V" is for Voges-Proskauer test, and "C" is for citrate test. The lower case "i" is merely for "in" as the Citrate test requires coliform samples to be placed "in Citrate". These tests are useful in distinguishing members of ...
The intensity of the colour correlates with the amount of the induced protein and thus genotoxic potency of the test sample. Quantitative metrics are used, with the absorbance of the plate being read at OD600 before and after the growth phase, as well as the OD420 after ONPG incubation. This allows for the calculation of the Induction Ratio (IR ...
P. hauseri is ONPG negative and PDA positive [citation needed]. Unlike the more commonly seen species of Proteus, P. hauseri is also able to convert tryptophan into indole, resulting in a positive indole test. P. hauseri shares a similar biochemical profile with Proteus vulgaris but can be differentiated by its ability to produce acid from ...
The spot indole test is used to determine if a microbe can deaminate tryptophan to produce indole. This test is performed by saturating a piece of filter paper with Indole Kovacs Reagent and scraping a portion of microbe onto the paper. A color to a pink-red color indicates a positive result while no color change indicates the lack of ...
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McFarland standards. No. 0.5, 1 and 2. In microbiology, McFarland standards are used as a reference to adjust the turbidity of bacterial suspensions so that the number of bacteria will be within a given range to standardize microbial testing.
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