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The most common types are the petri dish [1] [2] ... Penicillium mold colonies in a Petri dish: See also. Roux culture bottle; Inoculation loop; Test tube; References
The Petri dish was developed by German physician Julius Richard Petri (after whom the name is given) while working as an assistant to Robert Koch at Berlin University.Petri did not invent the culture dish himself; rather, it was a modified version of Koch's invention [9] which used an agar medium that was developed by Walther Hesse. [10]
An agar plate is a Petri dish that contains a growth medium solidified with agar, used to culture microorganisms. Sometimes selective compounds are added to influence growth, such as antibiotics. [1] 96 pinner used to perform spot assays with yeast, fungal or bacterial cells
Four Aspergillus species in a Petri dish. The bottom two are strains of A. oryzae. Various types of kōji are used, including yellow, black, and white. [2] [1] The kōji is stored for two to three days at 30 °C under high humidity to allow A. oryzae to grow. [3]
Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet.Thereafter, the tissue is grown in sterile containers, such as Petri dishes or flasks in a growth room with controlled temperature and light intensity.
In 1964, Ronald Hare took up the challenge. Like those before him, he found he could not get the mould to grow properly on a plate containing staphylococci colonies. He re-examined Fleming's paper and images of the original Petri dish. He attempted to replicate the original layout of the dish so there was a large space between the staphylococci.
Researchers of indoor mold also use a long-term settled dust collection system where a dust cloth or a petri dish is left out in the environment for a set period of time, sometimes weeks. [17] Dust samples can be analyzed using culture-based or culture-independent methods.
Colony-forming units are used to quantify results in many microbiological plating and counting methods, including: The pour plate method wherein the sample is suspended in a Petri dish using molten agar cooled to approximately 40–45 °C (just above the point of solidification to minimize heat-induced cell death).