Search results
Results from the WOW.Com Content Network
In biochemistry, denaturation is a process in which proteins or nucleic acids lose folded structure present in their native state due to various factors, including application of some external stress or compound, such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), agitation and radiation, or heat. [3]
A specially denatured alcohol is a combination of ethanol and another chemical substance, e.g., ethyl acetate in SDA 29, 35, and 35A, added to render the mixture unsuitable for drinking. [12] SDAs are often used in cosmetic products, and can also be used in chemical manufacturing, pharmaceuticals, and solvents. [ 13 ]
Denaturation (biochemistry), a structural change in macromolecules caused by extreme conditions; Denaturation (fissile materials), transforming fissile materials so that they cannot be used in nuclear weapons; Denaturation (food), intentional adulteration of food or drink rendering it unfit for consumption while remaining suitable for other uses
Chemical denaturation [ edit ] In the less extensive technique of equilibrium unfolding , the fractions of folded and unfolded molecules (denoted as p N {\displaystyle p_{N}} and p U {\displaystyle p_{U}} , respectively) are measured as the solution conditions are gradually changed from those favoring the native state to those favoring the ...
The process of DNA denaturation can be used to analyze some aspects of DNA. Because cytosine / guanine base-pairing is generally stronger than adenine / thymine base-pairing, the amount of cytosine and guanine in a genome is called its GC-content and can be estimated by measuring the temperature at which the genomic DNA melts. [ 2 ]
The hydrogen is dangerous and could ignite with the oxygen in the air, so the chemical procedure should be done in an inert atmosphere (e.g., nitrogen). Deprotonation can be an important step in a chemical reaction. Acid–base reactions typically occur faster than any other step which may determine the product of a reaction. The conjugate base ...
Enzyme denaturation is normally linked to temperatures above ... Substrate presentation is a process where the enzyme is sequestered ... Each chemical modification ...
The hyperchromic effect is the striking increase in absorbance of DNA upon denaturation. The two strands of DNA are bound together mainly by the stacking interactions, hydrogen bonds and hydrophobic effect between the complementary bases. The hydrogen bond limits the resonance of the aromatic ring so the absorbance of the sample is limited as well.