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  2. Denaturation (biochemistry) - Wikipedia

    en.wikipedia.org/wiki/Denaturation_(biochemistry)

    In biochemistry, denaturation is a process in which proteins or nucleic acids lose folded structure present in their native state due to various factors, including application of some external stress or compound, such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), agitation and radiation, or heat. [3]

  3. Equilibrium unfolding - Wikipedia

    en.wikipedia.org/wiki/Equilibrium_unfolding

    Equilibrium unfolding. In biochemistry, equilibrium unfolding is the process of unfolding a protein or RNA molecule by gradually changing its environment, such as by changing the temperature or pressure, pH, adding chemical denaturants, or applying force as with an atomic force microscope tip. [1][2] If the equilibrium was maintained at all ...

  4. Denatured alcohol - Wikipedia

    en.wikipedia.org/wiki/Denatured_alcohol

    A specially denatured alcohol is a combination of ethanol and another chemical substance, e.g., ethyl acetate in SDA 29, 35, and 35A, added to render the mixture unsuitable for drinking. [12] SDAs are often used in cosmetic products, and can also be used in chemical manufacturing, pharmaceuticals, and solvents. [ 13 ]

  5. Protein engineering - Wikipedia

    en.wikipedia.org/wiki/Protein_engineering

    Protein engineering. Protein engineering is the process of developing useful or valuable proteins through the design and production of unnatural polypeptides, often by altering amino acid sequences found in nature. [1] It is a young discipline, with much research taking place into the understanding of protein folding and recognition for protein ...

  6. Denaturation - Wikipedia

    en.wikipedia.org/wiki/Denaturation

    Denaturation (biochemistry), a structural change in macromolecules caused by extreme conditions. Denaturation (fissile materials), transforming fissile materials so that they cannot be used in nuclear weapons. Denaturation (food), intentional adulteration of food or drink rendering it unfit for consumption while remaining suitable for other uses.

  7. Southern blot - Wikipedia

    en.wikipedia.org/wiki/Southern_blot

    Southern blot is a method used for detection and quantification of a specific DNA sequence in DNA samples. This method is used in molecular biology. Briefly, purified DNA from a biological sample (such as blood or tissue) is digested with restriction enzymes, and the resulting DNA fragments are separated by electrophoresis using an electric ...

  8. Chemical reaction - Wikipedia

    en.wikipedia.org/wiki/Chemical_reaction

    A chemical reaction is a process that leads to the chemical transformation of one set of chemical substances to another. [1] When chemical reactions occur, the atoms are rearranged and the reaction is accompanied by an energy change as new products are generated. Classically, chemical reactions encompass changes that only involve the positions ...

  9. Superoxide dismutase - Wikipedia

    en.wikipedia.org/wiki/Superoxide_dismutase

    Irwin Fridovich and Joe McCord at Duke University discovered the enzymatic activity of superoxide dismutase in 1968. [5] SODs were previously known as a group of metalloproteins with unknown function; for example, CuZnSOD was known as erythrocuprein (or hemocuprein, or cytocuprein) or as the veterinary anti-inflammatory drug "Orgotein". [6]