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Diagnostic microbiology laboratories and reference laboratories are key for identifying outbreaks of MRSA. Normally, a bacterium must be cultured from blood, urine, sputum, or other body-fluid samples, and in sufficient quantities to perform confirmatory
First-line treatment for serious invasive infections due to MRSA is currently glycopeptide antibiotics (vancomycin and teicoplanin). A number of problems with these antibiotics occur, such as the need for intravenous administration (no oral preparation is available), toxicity, and the need to monitor drug levels regularly by blood tests.
As of 2017, point-of-care resistance diagnostics were available for methicillin-resistant Staphylococcus aureus (MRSA), rifampin-resistant Mycobacterium tuberculosis (TB), and vancomycin-resistant enterococci (VRE) through GeneXpert by molecular diagnostics company Cepheid. [41]
The diagnosis of vancomycin-resistant Staphylococcus aureus (VRSA) is performed by performing susceptibility testing on a single S. aureus isolate to vancomycin. This is accomplished by first assessing the isolate's minimum inhibitory concentration (MIC) using standard laboratory methods, including disc diffusion, gradient strip diffusion, and automated antimicrobial susceptibility testing ...
This is called a D-zone test, or D test. If a 'D' shape is formed around the clindamycin disk (distinguished from a circular zone of inhibition) then the isolate is reported as resistant to clindamycin. [5] This occurs due to erythromycin inducing the bacteria's erm gene, and thus making it resistant to clindamycin (MLS-B phenotype). [6] [7]
A slide coagulase test is run with a negative control to rule out autoagglutination. Two drops of saline are put onto the slide labeled with sample number, Test (T) and control (C). The two saline drops are emulsified with the test organism using a wire loop, straight wire, or wooden stick.
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A staphylococcal infection or staph infection is an infection caused by members of the Staphylococcus genus of bacteria.. These bacteria commonly inhabit the skin and nose where they are innocuous, but may enter the body through cuts or abrasions which may be nearly invisible.