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ATP is quantified by measuring the light produced through its reaction with the naturally occurring firefly enzyme luciferase using a luminometer. The amount of light produced is directly proportional to the amount of ATP present in the sample. ATP tests can be used to: Control biological treatment reactors; Guide biocide dosing programs
The Microtox Model 500 is a laboratory-based photometer that measures acute toxicity.This analyser is a temperature-controlled, self-calibrating biosensor measuring system that uses the bioluminescence of Allivibrio fischeri to determine the toxicity of contaminated water, or elutriates of contaminated soils and sediments.
Firefly luciferase is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles. The enzyme catalyses the oxidation of firefly luciferin, requiring oxygen and ATP. Because of the requirement of ATP, firefly luciferases have been used extensively in biotechnology.
In addition to using dyes to indicate the redox potential of cells in order to monitor their viability, researchers have developed assays that use ATP content as a marker of viability. [2] Such ATP-based assays include bioluminescent assays in which ATP is the limiting reagent for the luciferase reaction. [6]
A standard example of chemiluminescence in the laboratory setting is the luminol test. Here, blood is indicated by luminescence upon contact with iron in hemoglobin. When chemiluminescence takes place in living organisms, the phenomenon is called bioluminescence. A light stick emits light by chemiluminescence.
Bioluminescence is the emission of light during a chemiluminescence reaction by living organisms. [1] Bioluminescence occurs in diverse organisms ranging from marine vertebrates and invertebrates, as well as in some fungi, microorganisms including some bioluminescent bacteria, dinoflagellates and terrestrial arthropods such as fireflies.
The murine local lymph node assay (LLNA) is an in vivo test for skin sensitisation.. LLNA has largely superseded the guinea pig maximisation test and the Buehler test.It is considered more scientific and less cruel (lower number of animals; less suffering) and has found broad scientific and regulatory acceptance.
Imaging of engineered E. coli Nissle 1917 in the mouse gut. Bioluminescence imaging (BLI) is a technology developed over the past decades (1990's and onward). [1] [2] [3] [when?] that allows for the noninvasive study of ongoing biological processes [4] [1] [5] [6] [7] Recently, bioluminescence tomography (BLT) has become possible and several systems have become commercially available.
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