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ATP is a molecule found only in and around living cells, and as such it gives a direct measure of biological concentration and health. ATP is quantified by measuring the light produced through its reaction with the naturally-occurring firefly enzyme luciferase using a luminometer.
Firefly luciferase is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles. The enzyme catalyses the oxidation of firefly luciferin, requiring oxygen and ATP. Because of the requirement of ATP, firefly luciferases have been used extensively in biotechnology.
Bioluminescence is the emission of light during a chemiluminescence reaction by living organisms. [1] Bioluminescence occurs in diverse organisms ranging from marine vertebrates and invertebrates, as well as in some fungi, microorganisms including some bioluminescent bacteria, dinoflagellates and terrestrial arthropods such as fireflies.
Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein.The name was first used by Raphaël Dubois who invented the words luciferin and luciferase, for the substrate and enzyme, respectively. [1]
The Microtox Model 500 is a laboratory-based photometer that measures acute toxicity.This analyser is a temperature-controlled, self-calibrating biosensor measuring system that uses the bioluminescence of Allivibrio fischeri to determine the toxicity of contaminated water, or elutriates of contaminated soils and sediments.
A bioluminescent assay for ATP in water borne bacteria is made by adding nitric acid to a water sample with concentrated bacteria to rupture the bacterial cells. The sample is diluted with sterile, deionized water, then mixed with a luciferase-luciferin mixture and the resulting light output of the bioluminescent reaction is measured and ...
Let’s take a closer look at these 7 Bioluminescent Bugs, where they live, their colors, and much more! 1. Railroad Worms. Railroad worms have eleven pairs of luminescent organs.
Firefly luciferin (also known as beetle luciferin) is the luciferin, precursor of the light-emitting compound, used for the firefly , railroad worm (Phengodidae), starworm (Rhagophthalmidae), and click-beetle (Pyrophorini) bioluminescent systems. [1]
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