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In chemical thermodynamics, isothermal titration calorimetry (ITC) is a physical technique used to determine the thermodynamic parameters of interactions in solution. [ 1 ] [ 2 ] It is most often used to study the binding of small molecules (such as medicinal compounds) to larger macromolecules ( proteins , DNA etc.) in a label-free environment.
Calorimetry is the science of measuring the heat of chemical reactions or physical changes. Calorimetry is performed with a calorimeter.. Isothermal microcalorimetry (IMC) is a laboratory method for real-time, continuous measurement of the heat flow rate (μJ/s = μW) and cumulative amount of heat (J) consumed or produced at essentially constant temperature by a specimen placed in an IMC ...
A calorimeter is a device used for calorimetry, or the process of measuring the heat of chemical reactions or physical changes as well as heat capacity. Differential scanning calorimeters, isothermal micro calorimeters, titration calorimeters and accelerated rate calorimeters are among the most common types. A simple calorimeter just consists ...
Isothermal titration calorimeter An isothermal titration calorimeter uses the heat produced or consumed by the reaction to determine the equivalence point. This is important in biochemical titrations, such as the determination of how substrates bind to enzymes .
The thermodynamics of the host- guest interaction can be assessed by NMR spectroscopy, UV/visible spectroscopy, and isothermal titration calorimetry. [9] Quantitative analysis of binding constant values provides useful thermodynamic information. [10] An association constant, can be defined by the expression
The aggregation number of micelles can be determined by isothermal titration calorimetry when the aggregation number is not too high. [2] [3] Another classical experiment to determine the mean aggregation number would involve the use of a luminescent probe, a quencher and a known concentration of surfactant. If the concentration of the quencher ...
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