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An ANA test is considered positive if fluorescence is seen at a titre of 1:40/1:80. Higher titres are more clinically significant as low positives (≤1:160) are found in up to 20% of healthy individuals, especially the elderly. Only around 5% of the healthy population have ANA titres of 1:160 or higher. [8] [53]
HEp-2 cells provide a greater ability to differentiate patterns of ANA than animal sections, due to the large nuclei and high mitotic rate of the cell line. Upon incubation with serum containing anti-dsDNA antibodies and fluorescent labelled secondary antibodies, homogeneous staining of interphase nuclei and condensed chromosomal staining of ...
The ANA tests for the presence or absence of autoantibodies, while the ENA panel evaluates which proteins in the cell nucleus the autoantibodies recognize. The ENA panel helps diagnosis, distinguish between, and monitor the progression of autoimmune diseases and is performed with a simple blood draw.
Immunofluorescence pattern of SS-A and SS-B antibodies. Produced using serum from a patient on HEp-20-10 cells with a FITC conjugate. Anti-SSA autoantibodies (anti–Sjögren's-syndrome-related antigen A autoantibodies, also called anti-Ro, or similar names including anti-SSA/Ro, anti-Ro/SSA, anti–SS-A/Ro, and anti-Ro/SS-A) are a type of anti-nuclear autoantibodies that are associated with ...
The VEB IFA-Gelenkwellenwerk Haldensleben, known as "IFA-Gelenkwelle", was founded in 1959. [7] It was a supplier to the GDR vehicle and mechanical engineering industry, which belonged to the VEB IFA-Kombinat Nutzfahrzeuge Ludwigsfelde, which in turn was part of the Industrieverband Fahrzeugbau der DDR (IFA). [8]
Immunofluorescence (IF) on ethanol-fixed neutrophils is used to detect ANCA, although formalin-fixed neutrophils may be used to help differentiate ANCA patterns. ANCA can be divided into four patterns when visualised by IF; cytoplasmic ANCA (c-ANCA), C-ANCA (atypical), perinuclear ANCA (p-ANCA) and atypical ANCA (a-ANCA), also known as x-ANCA. c-ANCA shows cytoplasmic granular fluorescence ...
Hering–Breuer reflex — is a reflex triggered to prevent over-inflation of the lung; Hoffmann's reflex — also known as the finger flexor reflex; middle finger and thumb response. Test can indicate both neurological damage and nerve regeneration; often combined with the Babinski reflex test. Jaw jerk reflex
IFA’s exposure database MEGA provides data on hazardous chemicals gathered through atmospheric measurements and material analyses since 1972. It is determined to support the institutions for statutory accident insurance and prevention with epidemiological issues or retrospective considerations related to occupational diseases.