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Most contaminated cell lines are discarded; however, sometimes contaminant cells have acquired novel characteristics (e.g., by mutation or viral transfection, for example the HeLa derivate Det98) and thus constitute a truly novel lineage, so they are not thrown away. If a cell line is thought to be contaminated, it is usually tested for ...
Cell culture or tissue culture is the process by ... is the potential for contamination of the culture with ... for some cell types. For example, ...
Mycoplasma species are often found in research laboratories as contaminants in cell culture. Mycoplasmal cell culture contamination occurs due to contamination from individuals or contaminated cell culture medium ingredients. [34] Mycoplasma cells are physically small – less than 1 μm, so are difficult to detect with a conventional microscope.
Contamination on agar plate. In the biological sciences, accidental introduction of "foreign" material can seriously distort the results of experiments where small samples are used. In cases where the contaminant is a living microorganism, it can often multiply to dominate the sample and render it useless, as in contaminated cell culture lines.
HeLa cells are rapidly dividing cancer cells, and the number of chromosomes varies during cancer formation and cell culture. The current estimate (excluding very tiny fragments) is a "hypertriploid chromosome number (3n+)", which means 76 to 80 total chromosomes (rather than the normal diploid number of 46) with 22–25 clonally abnormal ...
Walter Nelson-Rees (January 11, 1929 – January 23, 2009) was a cell culture worker and cytogeneticist who helped expose the problem of cross-contamination of cell lines. He used chromosome banding to show that many immortal cell lines , previously thought to be unique, were actually HeLa cell lines .
A cell culture assay is any method used to assess the cytotoxicity of a material. [1] [2] This refers to the in vitro assessment of a material to determine whether it releases toxic chemicals in the cell. It also determines if the quantity is sufficient to kill cells, either directly or indirectly, through the inhibition of cell metabolic pathways.
A cell line can be defined as a permanently established cell culture which will propagate forever. Investigators mostly get cell lines from other investigators or from cell banks (such as the American Type Culture Collection) , because its much easier than creating new one. In special cases, investigators are obligated to establish a cell line.