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Gene length: Longer genes will have more fragments/reads/counts than shorter genes if transcript expression is the same. This is adjusted by dividing the FPM by the length of a feature (which can be a gene, transcript, or exon), resulting in the metric fragments per kilobase of feature per million mapped reads (FPKM). [90]
Sequencing technologies vary in the length of reads produced. Reads of length 20-40 base pairs (bp) are referred to as ultra-short. [2] Typical sequencers produce read lengths in the range of 100-500 bp. [3] However, Pacific Biosciences platforms produce read lengths of approximately 1500 bp. [4] Read length is a factor which can affect the results of biological studies. [5]
The entire set of fragments must be cloned together with the vector, and separation of clones can occur after. In either case, the fragments are ligated into a vector that has been digested with the same restriction enzyme. The vector containing the inserted fragments of genomic DNA can then be introduced into a host organism. [1]
2×10 3: UNIVAC I, first American commercially available electronic general-purpose stored program digital computer, 1951 [2] 3×10 3: PDP-1 commercial minicomputer, 1959 [2] 15×10 3: IBM Naval Ordnance Research Calculator, 1954; 24×10 3: AN/FSQ-7 Combat Direction Central, 1957 [2] 30×10 3: IBM 1130 commercial minicomputer, 1965 [2]
2 MHz Clarke (Saturn 1LT8 core) 32 KB RAM, not flashable 131×64 pixel monochrome LCD Entry RPN: Dynamic: RPL: Rudimentary [8] 8-bit RPL character set: Buzzer 1×4-pin RS-232, HP SIR: 3×1.5 V (4.5 V) AAA: 1991–1993 [11] HP-28S: HP 48G: HP 48SX: 2 MHz Clarke (Saturn 1LT8 core) 32 KB RAM, expandable via 2 card slots of 128 KB RAM/ROM each, not ...
There are two common methods in which to construct a DNA molecular-weight size marker. [3] One such method employs the technique of partial ligation. [3] DNA ligation is the process by which linear DNA pieces are connected to each other via covalent bonds; more specifically, these bonds are phosphodiester bonds. [4]
The level of unique gene expression is represented by the count of transcripts present per million molecules, similar to SAGE output. A significant advantage is the larger library size compared with SAGE. An MPSS library typically holds 1 million signature tags, which is roughly 20 times the size of a SAGE library.
Writing a DVD at 1× (1 385 000 bytes per second) [5] is approximately 9 times faster than writing a CD at 1× (153 600 bytes per second). [6] However, the actual speeds depend on the type of data being written to the disc. [6] For Blu-ray discs, 1× speed is defined as 36 megabits per second (Mbit/s), which is equal to 4.5 megabytes per second ...