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Sequencing technologies vary in the length of reads produced. Reads of length 20-40 base pairs (bp) are referred to as ultra-short. [2] Typical sequencers produce read lengths in the range of 100-500 bp. [3] However, Pacific Biosciences platforms produce read lengths of approximately 1500 bp. [4] Read length is a factor which can affect the results of biological studies. [5]
There are two common methods in which to construct a DNA molecular-weight size marker. [3] One such method employs the technique of partial ligation. [3] DNA ligation is the process by which linear DNA pieces are connected to each other via covalent bonds; more specifically, these bonds are phosphodiester bonds. [4]
In single-end sequencing, there is only one read per fragment (i.e., RPM = FPM). In paired-end sequencing, there are two reads per fragment ( i.e. , RPM = 2 x FPM). Sequencing depth is sometimes referred to as library size , the number of intermediary cDNA molecules in the experiment.
1×10 −1: multiplication of two 10-digit numbers by a 1940s electromechanical desk calculator [1] 3×10 −1: multiplication on Zuse Z3 and Z4, first programmable digital computers, 1941 and 1945 respectively; 5×10 −1: computing power of the average human mental calculation [clarification needed] for multiplication using pen and paper
A restriction fragment is a DNA fragment resulting from the cutting of a DNA strand by a restriction enzyme (restriction endonucleases), a process called restriction. [1] Each restriction enzyme is highly specific, recognising a particular short DNA sequence, or restriction site, and cutting both DNA strands at specific points within this site.
The level of unique gene expression is represented by the count of transcripts present per million molecules, similar to SAGE output. A significant advantage is the larger library size compared with SAGE. An MPSS library typically holds 1 million signature tags, which is roughly 20 times the size of a SAGE library.
Using Illumina GA sequence data and SOAPdenovo, a de Bruijn graph-based tool specifically designed for assembly short reads, they were able to generate 6.58 million contigs greater than 500 bp for a total contig length of 10.3 Gb and a N50 length of 2.2 kb.
2 −1: 10 −1: 0.415 bits (log 2 4/3) – amount of information needed to eliminate one option out of four. 0.6–1.3 bits – approximate information per letter of English text. [3] 2 0: bit: 10 0: bit 1 bit – 0 or 1, false or true, Low or High (a.k.a. unibit) 1.442695 bits (log 2 e) – approximate size of a nat (a unit of information ...