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S. cerevisiae septins revealed with fluorescent microscopy utilizing fluorescent labeling. In molecular biology and biotechnology, a fluorescent tag, also known as a fluorescent label or fluorescent probe, is a molecule that is attached chemically to aid in the detection of a biomolecule such as a protein, antibody, or amino acid.
Laser-induced fluorescence (LIF) or laser-stimulated fluorescence (LSF) [1] is a spectroscopic method in which an atom or molecule is excited to a higher energy level by the absorption of laser light followed by spontaneous emission of light. [2] [3] It was first reported by Zare and coworkers in 1968. [4] [5]
The fluorophore absorbs light energy of a specific wavelength and re-emits light at a longer wavelength. The absorbed wavelengths, energy transfer efficiency, and time before emission depend on both the fluorophore structure and its chemical environment, since the molecule in its excited state interacts with surrounding molecules.
Some of the widely used laser sources are Nd:YAG laser, dye lasers, excimer lasers, and ion lasers. The light from the laser (usually a beam) is passed through a set of lenses and/or mirrors to form a sheet, which is then used to illuminate the medium. This medium is either made up of fluorescent material or can be seeded with a fluorescent ...
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Average: 4.7 out of 5 - 83,309 reviews - Power Reviews