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TCEP is often used as a reducing agent to break disulfide bonds within and between proteins as a preparatory step for gel electrophoresis.. Compared to the other two most common agents used for this purpose (dithiothreitol and β-mercaptoethanol), TCEP has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent (in the sense that TCEP does not ...
Dithiobutylamine (DTBA) is a reducing agent intended as an alternative for DTT in biochemical uses. It was designed to be easily synthesized in non-racemic form, to have a lower pKa (allowing more effective reduction at neutral pH), and to have a low disulfide E°′ reduction potential. [1]
The upper part of the valve stem, within the rocker box, is lubricated by oil. If this oil travels unchecked along the valve stem, engine HC emissions will become excessive. To control this, an elastomeric seal is fitted over the top of the valve guide. These may wear or stiffen with age, so are usually replaced whenever valves are removed for ...
Dithiothreitol (DTT) is an organosulfur compound with the formula (CH(OH)CH 2 SH) 2. A colorless compound, it is classified as a dithiol and a diol . DTT is redox reagent also known as Cleland's reagent , after W. Wallace Cleland . [ 2 ]
2-Mercaptoethanol (also β-mercaptoethanol, BME, 2BME, 2-ME or β-met) is the chemical compound with the formula HOCH 2 CH 2 SH. ME or βME, as it is commonly abbreviated, is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals (amongst others).
A valve stem is a self-contained valve that opens to admit gas to a chamber (such as air to inflate a tire), [1] and is then automatically closed and kept sealed by the pressure in the chamber, or a spring, [2] [3] or both, to prevent the gas from escaping.
Biomedical engineering (BME) or medical engineering is the application of engineering principles and design concepts to medicine and biology for healthcare applications (e.g., diagnostic or therapeutic purposes). BME is also traditionally logical sciences to advance health care treatment, including diagnosis, monitoring, and therapy.
Internal parts of a typical globe valve. The body is the main pressure-containing structure of the valve and the most easily identified as it forms the mass of the valve.It contains all of the valve's internal parts that will come in contact with the substance being controlled by the valve.