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Immunodiffusion is a laboratory technique used to detect and quantify antigens and antibodies by observing their interactions within a gel medium. [1] This technique involves the diffusion of antigens and antibodies through a gel, usually agar, resulting in the formation of a visible precipitate when they interact.
In immunology the particular macromolecule bound by an antibody is referred to as an antigen and the area on an antigen to which the antibody binds is called an epitope. In some cases, an immunoassay may use an antigen to detect for the presence of antibodies, which recognize that antigen, in a solution.
In the past nucleic acid tests have mainly been used as a secondary test to confirm positive serological results. [3] However, as they become cheaper and more automated, they are increasingly becoming the primary tool for diagnostics and can also be use for monitoring of treatment of viral infected individuals t.
Handbook of Experimental Immunology. Vol. 1. Immunochemistry (4th ed.). Oxford, England: Blackwell Scientific. pp. 32.1 – 32.50. ISBN 9780632014996 at Google Books "Ouchterlony Analysis" (PDF). Medical Immunology 544. University of California, Irvine College of Medicine. Fall 2011. Archived from the original (PDF) on 2012-02-07
Archived from the original (PDF) on 2017-08-08; LSUMC/MIP Dental Microbiology Lab (2002). "II. Lab Work: B. Radial Immunodiffusion". Exercise 3: Antigen-Antibody I. New Orleans, Louisiana: Louisiana State University School of Medicine: Department of Microbiology, Immunology & Parasitology. Archived from the original on 2004-08-04