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A polarimeter [1] is a scientific instrument used to measure optical rotation: the angle of rotation caused by passing linearly polarized light through an optically active substance. [ 2 ] Some chemical substances are optically active, and linearly polarized (uni-directional) light will rotate either to the left (counter-clockwise) or right ...
Optical activity is measured using a polarized source and polarimeter. This is a tool particularly used in the sugar industry to measure the sugar concentration of syrup, and generally in chemistry to measure the concentration or enantiomeric ratio of chiral molecules in solution.
A simple polarimeter to measure this rotation consists of a long tube with flat glass ends, into which the sample is placed. At each end of the tube is a Nicol prism or other polarizer. Light is shone through the tube, and the prism at the other end, attached to an eye-piece, is rotated to arrive at the region of complete brightness or that of ...
Recording optical rotation with a polarimeter: The plane of polarisation of plane polarised light (4) rotates (6) as it passes through an optically active sample (5). This angle is determined with a rotatable polarizing filter (7). In chemistry, specific rotation ([α]) is a property of a chiral chemical compound.
In order for a molecule (or crystal) to exhibit circular birefringence and circular dichroism, it must be distinguishable from its mirror image.An object that cannot be superimposed on its mirror image is said to be chiral, and optical rotatory dispersion and circular dichroism are known as chiroptical properties.
Michael Faraday holding a piece of glass of the type he used to demonstrate the effect of magnetism on polarization of light, c. 1857.. By 1845, it was known through the work of Augustin-Jean Fresnel, Étienne-Louis Malus, and others that different materials are able to modify the direction of polarization of light when appropriately oriented, [4] making polarized light a very powerful tool to ...
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Nicol prisms were once widely used in mineralogical microscopy and polarimetry, and the term "using crossed Nicols" (abbreviated as XN) is still used to refer to the observing of a sample placed between orthogonally oriented polarizers.