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Ribotyping is a molecular technique for bacterial identification and characterization that uses information from rRNA-based phylogenetic analyses. [1] It is a rapid and specific method widely used in clinical diagnostics and analysis of microbial communities in food, water, and beverages.
Bacterial 16S ribosomal RNA, 23S ribosomal RNA, and 5S rRNA genes are typically organized as a co-transcribed operon. As shown by the image in this section, there is an internal transcribed spacer between 16S and 23S rRNA genes . [ 28 ]
This is a view of the 3D arrangement of the 23S and 5S rRNA in the Escherichia coli 50S ribosomal subunit based on a cryo-electron microscopic reconstruction. [1] The 23S rRNA is a 2,904 nucleotide long (in E. coli) component of the large subunit of the bacterial/archean ribosome and makes up the peptidyl transferase center (PTC). [2]
Ribosomal RNA (rRNA) intergenic spacer analysis (RISA) is a method of microbial community analysis that provides a means of comparing differing environments or treatment impacts without the bias imposed by culture- dependent approaches. [1]
In bacteria and archaea, there is a single ITS, located between the 16S and 23S rRNA genes. Conversely, there are two ITSs in eukaryotes: ITS1 is located between 18S and 5.8S rRNA genes, while ITS2 is between 5.8S and 28S (in opisthokonts, or 25S in plants) rRNA genes. ITS1 corresponds to the ITS in bacteria and archaea, while ITS2 originated ...
Small subunit ribosomal ribonucleic acid (SSU rRNA) is the smaller of the two major RNA components of the ribosome. Associated with a number of ribosomal proteins , the SSU rRNA forms the small subunit of the ribosome.
In the cytoplasm, ribosomal RNA and protein combine to form a nucleoprotein called a ribosome. The ribosome binds mRNA and carries out protein synthesis. Several ribosomes may be attached to a single mRNA at any time. [27] Nearly all the RNA found in a typical eukaryotic cell is rRNA. Transfer-messenger RNA (tmRNA) is found in many bacteria and ...
ribosomal RNA multiple families: 5S rRNA: 5S ribosomal RNA RF00001: 5.8S rRNA: 5.8S ribosomal RNA RF00002: SSU rRNA: small subunit ribosomal RNA CL00111: LSU rRNA: large subunit ribosomal RNA CL00112: NoRC RNA: nucleolar remodeling complex associated RNA RF01518: pRNA promoter RNA RF01518: synonym of NoRC RNA: 6S RNA: 6S RNA RF00013: SsrS RNA ...
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