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The slide that has been treated with diastase will lack any positive PAS staining in those locations on the slide PAS staining is also used for staining cellulose. One example would be looking for implanted medical devices composed of nonoxidized cellulose. If the PAS stain will be performed on tissue, the recommended fixative is 10% neutral ...
PAS diastase stain is also used to identify alpha-1 antitrypsin globules in hepatocytes, which is a characteristic finding of alpha-1 antitrypsin deficiency. [2] PAS diastase stain is also used in diagnosing Whipple’s disease , as the foamy macrophages that infiltrate the lamina propria of the small intestine in this disease possess PAS ...
Immunoperoxidase is a type of immunostain used in molecular biology, medical research, and clinical diagnostics.In particular, immunoperoxidase reactions refer to a sub-class of immunohistochemical or immunocytochemical procedures in which the antibodies are visualized via a peroxidase-catalyzed reaction.
Supravital stain of a smear of human blood from a patient with hemolytic anemia. The reticulocytes are the cells with the dark blue dots and curved linear structures (reticulum) in the cytoplasm. Supravital staining is a method of staining used in microscopy to examine living cells that
In 1960 he teamed with Dr. Robert Mowry to write Staining Methods; a handbook integrating newer histological and histochemical methods of tissue examination into standard laboratory procedures. After a one-year sabbatical in Oxford in 1960 working back at the same lab where he had made his earlier discovery, McManus accepted a teaching position ...
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue.
The Ziehl-Neelsen stain is a two step staining process. In the first step, the tissue is stained with a basic fuchsin solution, which stains all cells pink. In the second step, the tissue is incubated in an acid alcohol solution, which decolorizes all cells except for acid-fast cells, which retain the color and appeared as red.
Immunofluorescence is a widely used example of immunostaining (using antibodies to stain proteins) and is a specific example of immunohistochemistry (the use of the antibody-antigen relationship in tissues). This technique primarily utilizes fluorophores to visualize the location of the antibodies, while others provoke a color change in the ...